论文部分内容阅读
目的 :构建反义转录元件结合蛋白 2 (BTEB2 )重组腺病毒载体并研究BTEB2反义RNA对动脉损伤后新生内膜增生的影响。方法 :通过聚合酶链反应 (RT PCR)法从培养的大鼠血管平滑肌细胞中制备BTEB2cDNA ,将其反向克隆至腺病毒载体 ,构建反义BTEB2重组腺病毒 ;用重组腺病毒局部转染球囊损伤的大鼠颈动脉 ,观察反义BTEB2基因转染对BTEB2蛋白表达及损伤动脉新生内膜形成的影响。结果 :构建的BTEB2反义重组腺病毒经鉴定正确 ,其滴度为 5× 10 9/ml;反义BTEB2重组腺病毒转染可明显抑制BTEB2蛋白表达及新生内膜增生。结论 :成功构建了反义BTEB2重组腺病毒载体 ;BTEB2反义RNA可明显抑制大鼠颈动脉球囊损伤后的新生内膜增生。
Objective: To construct antisense transcription factor binding protein 2 (BTEB2) recombinant adenovirus vector and study the effect of BTEB2 antisense RNA on neointimal hyperplasia after arterial injury. METHODS: BTEB2 cDNA was prepared from cultured rat vascular smooth muscle cells by polymerase chain reaction (RT PCR), and then was reverse cloned into adenoviral vector to construct antisense BTEB2 recombinant adenovirus. The recombinant adenovirus was used to transfect spheroids To investigate the effect of transfection of antisense BTEB2 gene on the expression of BTEB2 protein and the neointimal hyperplasia of injured arteries in rats. Results: The constructed BTEB2 antisense recombinant adenovirus was identified correctly with a titer of 5 × 10 9 / ml. Transfection with antisense BTEB2 recombinant adenovirus significantly inhibited BTEB2 protein expression and neointimal hyperplasia. CONCLUSION: Antisense BTEB2 recombinant adenovirus vector was successfully constructed. BTEB2 antisense RNA could significantly inhibit neointimal hyperplasia after carotid artery balloon injury in rats.