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目的探讨用逆转录病毒载体转入 m IL - 3基因的基质细胞系 QXMSC1对骨髓造血细胞前体的影响。方法用含鼠 IL - 3基因的逆转录病毒载体感染骨髓基质细胞系 QXMSC1,获得 IL - 3高表达细胞系 QXMSC1IL - 3用于实验。观察QXMSC1IL - 3细胞上清对骨髓前体细胞 CFU- GM、CFU - E、CFU - GEMM的影响 ,QXMSC1IL - 3基质细胞对长期培养起始细胞的影响。结果 QXMSC1IL- 3培养上清对骨髓前体细胞、CFU- GM、CFU- E、CFU- GEMM有明显促进作用。QXMSC1IL- 3能明显增加有核细胞数和长期培养起始细胞数 ,诱导分化形成 CFU- GM增多。阻断基质细胞与造血细胞相互接触 ,QXMSC1IL- 3促造血细胞增殖作用有所减弱。结论基质细胞 QXMSC1IL- 3可能作为有效的基因载体进一步促进骨髓移植后或辐射损伤后的造血和免疫功能重建。
Objective To investigate the effect of QXMSC1, a stromal cell line transfected with mIL - 3 gene by retroviral vector, on bone marrow hematopoietic progenitor cells. Methods The murine IL - 3 gene - containing retroviral vector was used to infect the bone marrow stromal cell line QXMSC1 and the IL - 3 highly expressed cell line QXMSC1IL - 3 was used in the experiment. The effects of QXMSC1IL - 3 cells supernatant on CFU - GM, CFU - E and CFU - GEMM of bone marrow progenitor cells and the effect of QXMSC1IL - 3 stromal cells on long term culture initiating cells were observed. Results The culture supernatant of QXMSC1IL-3 could promote the proliferation of bone marrow progenitor cells, CFU-GM, CFU-E and CFU-GEMM. QXMSC1IL-3 can significantly increase the number of nucleated cells and long-term culture initiating cells, inducing differentiation to form CFU-GM increased. Blocked stromal cells and hematopoietic cells in contact with each other, QXMSC1IL- 3 promote hematopoietic cell proliferation has been reduced. Conclusion Stromal cells QXMSC1IL-3 may serve as an effective gene carrier to further promote hematopoietic and immune function reconstruction after bone marrow transplantation or radiation injury.