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目的探讨细胞因子白细胞介素-2(IL-2)、IL-8、肿瘤坏死因子-α(TNF-α)及一氧化氮(NO)对白细胞精子症的影响。方法120例不育症患者,按其白细胞(WBC)数量的多少,将其分为WBC精浆组46例(精液中WBC≥4/HP),非WBC精浆组74例(精液中WBC<4/HP),正常生育组50例。细胞因子IL-2、IL-8、TNF-α应用化学发光免疫分析技术检测;NO采用硝酸还原酶法进行检测。结果WBC精浆组IL-2、IL-8、TNF-α及NO水平分别为(1.18±1.04)、(3.55±0.85)、(5.22±1.56)ng/mL和(106.6±2.5)umim/L显著高于非WBC精浆组(0.67±0.57)、(2.63±0.85)、(4.43±1.77)ng/mL和(85.8±2.3)umim/L以及正常生育组(0.42±0.23)、(2.24±0.83)、(2.96±1.15)ng/mL和(45.8±1.5)umim/L,差异有统计学意义(P<0.01);非WBC精浆组与正常生育组相比,IL-2、IL-8、TNF-α及NO水平差异也有统计学意义(P<0.05)。结论高水平的IL-2、IL-8、TNF-α及NO在WBC精子症所致的不育症中发挥重要作用,IL诱导NO生成对精子和生殖组织具有毒性和损害作用而致男性不育。
Objective To investigate the effects of interleukin-2 (IL-2), interleukin-8, tumor necrosis factor-α (TNF-α) and nitric oxide (NO) on leukocyte spermatogenesis. Methods According to the number of white blood cells (WBC) in 120 infertile patients, 46 WBC seminal plasma samples (WBC≥4 / HP in semen) and 74 non-WBC seminal plasma samples (WBC < 4 / HP), normal birth group 50 cases. Cytokines IL-2, IL-8 and TNF-α were detected by chemiluminescence immunoassay. NO was detected by nitrate reductase method. Results The levels of IL-2, IL-8, TNF-αand NO in WBC seminal plasma were (1.18 ± 1.04), (3.55 ± 0.85), (5.22 ± 1.56) ng / mL and (106.6 ± 2.5) umim / (0.67 ± 0.57), (2.63 ± 0.85), (4.43 ± 1.77) ng / mL and (85.8 ± 2.3) umim / L in non-WBC seminal plasma and 0.42 ± 0.23 in normal fertility group (2.24 ± (2.96 ± 1.15) ng / mL and (45.8 ± 1.5) umim / L, respectively (P <0.01). Compared with normal fertility group, the levels of IL-2 and IL- 8, TNF-α and NO levels were also significantly different (P <0.05). Conclusions High levels of IL-2, IL-8, TNF-α and NO play an important role in the infertility caused by WBC spermatozoa. IL-induced NO production is toxic and damaging to sperm and reproductive tissues but not to men Education.