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对沙糖橘(Citrus reticulata Blancdo‘Shiyueju’)果实进行苯并噻二唑(S-methyl benzo thiadiazole-7-carbothioate,BTH)渗透处理后接种指状青霉菌孢子,12h后即可明显诱导果皮中抗病相关蛋白CR-CT1和CR-BG3基因表达并在贮藏期间维持较高水平;不同浓度BTH处理果实的发病率和病斑面积均比对照低,其中以0.025g·L-1BTH处理的效果最好;BTH处理提高了果皮几丁质酶(CT)、β–1,3–葡聚糖酶(GUN)、苯丙氨酸解氨酶(PAL)、多酚氧化酶(PPO)、过氧化物酶(POD)活性和H2O2含量,表明BTH诱导的沙糖橘果实抗病性与果实CR-CT1和CR-BG3基因的表达增强,CT、GUN、PAL、PPO和POD等相关酶活性和H2O2含量提高密切相关。
Inoculation of Penicillium expansum spores with S-methyl benzo thiadiazole-7-carbothioate (BTH) infiltration treatment on the fruit of Citrus reticulata Blancdo’Shiyueju ’ The expression of disease-related proteins CR-CT1 and CR-BG3 were maintained at a high level during storage. The morbidity and lesion area of fruits treated with different concentrations of BTH were lower than those of the control, and the effects of treatment with 0.025g · L-1BTH The best; BTH treatment increased pericarp chitinase (CT), β-1,3-glucanase (GUN), phenylalanine ammonia lyase (PAL), polyphenol oxidase (PPO) The activities of oxidase (POD) and H2O2 showed that BTH-induced resistance of Sittia citri fructus and the expression of CR-CT1 and CR-BG3 genes, CT, GUN, PAL, PPO and POD, H2O2 content is closely related.