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目的:研究甘肃地区汉族妇女XRCC1 rs25487、CCNH rs2234942基因多态性与乳腺癌及乳腺良性肿瘤发病的相关性。方法:选取经病理组织学确诊的乳腺癌、乳腺良性肿瘤各101例,匹配相同数量健康人作对照。使用聚合酶链式反应-限制性片段长度多态分析技术(PCR-RFLP)对XRCC1、CCNH进行基因型分析,通过Logistic回归分析不同基因型和临床病理特征与乳腺癌发病2的风险性关系,通过检验比较两种基因位点不同基因型的初潮年龄、发病年龄与乳腺癌和乳腺良性肿瘤的相关性。结果:Logistic回归分析发现XRCC1 rs25487位点GG基因型携带者的妇女罹患乳腺癌的危险性增加(P=0.001,OR=6.39,95%CI:2.18~+-18.65);临床病理免疫组化分析显示,XRCC1基因rs25487位点携带AA/AG基因型者,在PR与PR乳腺癌组织间的分布差异有显著+-2性(P=0.04,OR=0.29);携带AG/GG基因型者,在Her-2与Her-2乳腺癌组织间的分布差异有显著性(P=0.008,OR=0.45)。检验显示,乳腺癌和乳腺良性肿瘤患者XRCC1 rs25487位点GG/AG基因型携带者的初潮年龄差异有显著性(P=0.001、0.043);乳腺癌和乳腺良性肿瘤患者CCNH rs2234942位点GG基因型携带者的初潮年龄差异有显著性(P=0.049);乳腺癌和乳腺良性肿瘤患者XRCC1rs25487和CCNH rs2234942位点GG基因型携带者,发病年龄差异有显著性(P=0.019、0.048)。结论:XRCC1 rs25487 GG基因型将会+增加乳腺癌的发病风险,XRCC1 rs25487位点携带AA/AG基因型者,在PR时乳腺癌的发病风险下降;携带AG/GG基因型者,在+Her-2时可能降低乳腺癌的发病风险。
Objective: To investigate the association between the polymorphisms of XRCC1 rs25487 and CCNH rs2234942 and the incidence of breast cancer and benign breast neoplasm in Gansu Han women. Methods: 101 cases of breast cancer and benign breast tumor diagnosed by histopathology were selected, matched with the same number of healthy people as control. The genotypes of XRCC1 and CCNH were analyzed by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP). Logistic regression was used to analyze the relationship between different genotypes and clinicopathological features and risk of breast cancer. Through the test to compare the two genotypes of different genotypes of menarche age, age of onset and breast cancer and benign breast tumor correlation. Results: Logistic regression analysis showed that women with GG genotype at XRCC1 rs25487 site had a higher risk of developing breast cancer (P = 0.001, OR = 6.39, 95% CI: 2.18 ~ + -18.65). Clinicopathological immunohistochemistry The results showed that there was a significant + -2 (P = 0.04, OR = 0.29) difference in the distribution of PR / PR breast cancer among individuals with rs25487 locus carrying the AA / AG genotype of the XRCC1 gene. In patients with AG / GG genotype, The distribution of Her-2 and Her-2 breast cancer was significantly different (P = 0.008, OR = 0.45). The test showed that there was significant difference in the menarche age between XRCC1 rs25487 locus and breast cancer patients (P = 0.001,0.043). The GG genotype of CCNH rs2234942 locus in breast cancer and benign breast tumor patients (P = 0.049). The carriers of GG genotypes in XRCC1rs25487 and CCNH rs2234942 sites of breast cancer and benign breast tumor patients had significant difference in age of onset (P = 0.019,0.048). Conclusion: The genotype of XRCC1 rs25487 GG will increase the risk of breast cancer. The genotype of XRCC1 rs25487 carries AA / AG genotype, and the risk of breast cancer is decreased in PR. The genotype of AG / GG in + -2 may reduce the risk of breast cancer.