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目的探讨腺瘤样结肠息肉易感基因(APC)启动子区CpG甲基化及环境危险因素及相互作用与中国人群中前列腺癌(PCa)发病之间的关系。方法应用亚硫酸盐修饰后测序法检测60例PCa及40例良性前列腺增生(BPH)组织中APC基因启动子区CpG甲基化情况。分析APC基因甲基化及环境危险因素与PCa患病之间的关系。结果 APC基因在PCa和BPH组织中CG位点甲基化率分别为48.84%和1.19%;基因甲基化率与前列腺特异性抗原(PSA)、Gleason评分、病理分期和PCa危险分期之间关系密切;饮茶者患PCa的危险性是不饮茶者的0.29倍。结论APC基因启动子区甲基化与PCa发生及发展有关;其甲基化率的变化与PCa的危险分期关系密切。检测前列腺组织中相关基因甲基化状态,有望成为诊断早期PCa的一种方法。
Objective To investigate the relationship between CpG methylation in promoter region of adeno-associated colorectal polyps susceptibility gene (APC), environmental risk factors and their interaction with the incidence of prostate cancer (PCa) in Chinese population. Methods The methylation status of APC promoter in 60 cases of PCa and 40 cases of benign prostatic hyperplasia (BPH) was detected by sulfite modified sequencing. To analyze the relationship between APC gene methylation and environmental risk factors and PCa prevalence. Results The methylation rates of APC gene in PCa and BPH tissues were 48.84% and 1.19% respectively. The relationship between methylation rate and PSA, Gleason score, pathological staging and PCa risk staging Close; tea drinkers risk of PCa is 0.29 times than those who do not drink tea. Conclusion The promoter methylation of APC gene is related to the occurrence and development of PCa. The methylation rate of APC gene is closely related to the risk stage of PCa. Detecting the methylation status of related genes in prostate tissue is expected to become a method to diagnose early PCa.