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目的:试图观察髓鞘蛋白脂质蛋白多肽(proteolipidprotein,PLP)两种不同肽段PLP193～151和PLP178～191抗原决定簇对实验性自身免疫性脑脊髓炎(experimentalautoimmuneencephalomyelitis,EAE)模型建立的影响。方法:实验于2004-02/06在解放军总医院神经病理研究室进行。应用PLP193～151和PLP178～191免疫雌性SJL/J小鼠,制作复发缓解型实验性自身免疫性脑脊髓炎(relapseremittingEAE,RR-EAE)模型,观察其神经功能评分的变化及组织形态学改变;用不同浓度的两种肽段体外刺激经两种肽段免疫后的小鼠的淋巴细胞,并观察其增殖强度。结果:两种PLP肽段免疫的小鼠发病均具有缓解-复发的特点,并出现血管鞘形成、炎性细胞浸润及脱髓鞘等特征性改变;但是,PLP178～191诱导的模型发病时间早(P<0.01),症状较重,表现多样。不同PLP肽段及其浓度间的差异有统计学意义(P=0.000)。PLP178～191在5mg/L时A值最高(2.36±0.14),而PLP139～151在50和100mg/L的A值增高有统计学意义(P=0.000)。结论:PLP两种肽段均可诱发RR-EAE模型,但二者诱发的模型各具特点,这是由其不同抗原决定簇决定的。
Objective: To investigate the effects of PLP193 ~ 151 and PLP178 ~ 191 epitopes on the experimental autoimmune encephalomyelitis (EAE) in experimental autoimmune encephalomyelitis (EAE) model in mice with proteolipid protein (PLP). Methods: The experiment was performed in the Department of Neuropathology, Chinese PLA General Hospital from February to June 2004. Female SJL / J mice were immunized with PLP193 ~ 151 and PLP178 ~ 191, and the relapseremittingEAE (RR-EAE) model was established to observe the changes of neurological function scores and histomorphological changes. The lymphocytes of mice immunized with the two kinds of peptides were stimulated in vitro with different concentrations of two peptides and their proliferation intensity was observed. Results: The mice immunized with two kinds of PLP peptides all had the characteristics of remission and recurrence, and showed characteristic changes such as vascular sheath formation, inflammatory cell infiltration and demyelination; however, the onset time of PLP178 ~ 191 induced model was earlier (P <0.01), the symptoms were severe and the performance varied. The difference between different PLP peptides and their concentrations was statistically significant (P = 0.000). The highest A value (2.36 ± 0.14) for PLP178-151 at 5 mg / L, and the A value for PLP139-151 at 50 and 100 mg / L were statistically significant (P = 0.000). CONCLUSION: The two peptides of PLP can induce RR-EAE model, but the models induced by both of them have their own characteristics, which are determined by their different antigenic determinants.