流感嗜血菌及嗜肺军团菌(Legionella pneumophila)等细菌在某种程度上有抵抗巨噬细胞杀灭的能力,甚至能在巨噬细胞内增殖。因此,抗生素治疗这类细菌性感染时,其疗效可能与药物透入细胞的能力有关。本文以放射性同位素标记〔~(14)C〕-角沙霉素和〔~(14)C〕-青霉素G作为药物模型,研究了人体巨噬细胞对药物摄取的能力与细胞内药物抗菌作用之间的关系。将人体巨噬细胞(多形核白细胞、单核细胞和泡状巨噬细胞)分离出来,分别加入上述抗生素,37℃培养45分钟,试验结果,细胞内摄取药量与细胞外药量的比值(C/E)列于表一。从表一可见,角沙霉素容易透入人体巨噬细胞,在这些细胞中,角沙霉素的浓度大 Bacteria such as Haemophilus influenzae and Legionella pneumophila are somewhat resistant to macrophage killing and even proliferate in macrophages. Therefore, the antibiotic treatment of such bacterial infections, its efficacy may be related to the drug’s ability to penetrate cells. In this paper, radiolabelling [~ (14) C] -corbomycin and [~ (14) C] -penicillin G were used as drug models to study the ability of human macrophages to ingest drugs and the antibacterial activity of intracellular drugs Relationship between. Human macrophages (polymorphonuclear leucocytes, monocytes and vesicular macrophages) were isolated, and the above antibiotics were separately added and cultured at 37 ° C for 45 minutes. The results, the ratio of intracellular and extracellular doses (C / E) is listed in table I. As can be seen from Table 1, rhinocercus is easy to penetrate into human macrophages. In these cells, the concentration of tachognogamycin is large