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用６０只Ｗｉｓｔａｒ健康大鼠制作急性肠绞窄动物模型，根据肠绞窄不同时间，绞窄肠袢不同长度分别设立对照组及实验组，在大体及显微镜下观察肠壁组织学损害程度，并用７５１分光光度仪测定血清及腹水中碱性磷酸酶（ＡＫＰ）含量，结果发现随着肠壁组织损害程度的加重，腹水及血清中ＡＫＰ含量也逐渐升高。而腹水中ＡＫＰ含量变化较血清ＡＫＰ变化早且明显。本实验表明测定腹水及血清ＡＫＰ有助于判断肠绞窄时肠道缺血的状况。
Sixty Wistar healthy rats were used to make animal model of acute intestinal strangulation. According to the different lengths of intestinal strangulation, the control group and experimental group were set up according to different lengths of intestinal strangulation. The degree of intestinal histological damage was observed under microscope and under microscope 751 spectrophotometer determination of serum and ascites in alkaline phosphatase (AKP) content and found that as the degree of damage to the intestinal wall tissue, ascites and serum AKP content gradually increased. The ascites AKP content changes earlier and more obvious than serum AKP. This experiment shows that the determination of ascites and serum AKP help determine intestinal ischemia when intestinal strangulation.