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目的:明确1株分离于感病苹果株系群体的供试菌株AML0801的种类,探讨其生物学特性,为苹果抗病分子育种研究奠定基础。方法:通过形态特征描述,显微形态、超微结构观察等方法,从形态学上明确AML0801的分类。室内模拟侵染试验,了解AML0801致病性。ITS序列测定分析辅助验证供试菌株AML0801。结果:菌株AML0801的分生孢子、产孢表型等形态特征,符合苹果链格孢(Alternaria mali Roberts,A.mali)种的描述,ITS序列分析表明,AML0801与A.mali标准菌株同源性达99%。通过比较病原菌侵染试验证实AML0801具备一定的致病性。结论:结合供试菌株AML0801形态特征、ITS序列分析结果,以及室内模拟的致病性研究,可将供试菌株AML0801鉴定为苹果链格孢(A.mali)。
OBJECTIVE: To determine the type of AML0801 isolates from susceptible apple strains and explore the biological characteristics of them. This study lays the foundation for the research of molecular breeding of disease-resistant apple. Methods: The morphology of AML0801 was identified by morphological characterization, microscopic morphology and ultrastructure observation. Indoor simulated infection test to understand AML0801 pathogenicity. ITS Sequence Assay Assisted Validation Test strain AML0801. Results: The conidia and sporulation phenotypes of strain AML0801 were consistent with those of Alternaria mali Roberts (A.mali). ITS sequence analysis indicated that the homology of AML0801 with A.mali Up to 99%. By comparing the pathogen infection test confirmed that AML0801 has a certain pathogenicity. Conclusion: According to the morphological characteristics of the tested strain AML0801, ITS sequence analysis and pathogenicity test in laboratory, AML0801 can be identified as A.mali.