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目的观察梅毒患者驱梅治疗前、后外周血单一核细胞(PBMC)表面Toll样受体2的变化情况。方法对初诊为梅毒的患者分别于驱梅治疗前及驱梅治疗结束后1年抽取外周静脉血检测其PBMC的TLR2+CD14+表达情况及TLR2+CD14+占CD14+细胞群的比例。并选60例健康者作为对照组进行比较。结果初诊时梅毒患者外周血PBMC的中CD14+TLR2+表达及百分比均明显低于正常对照组,且差异均有统计学意义(P<0.001)。其中一期的初诊梅毒患者外周血PBMC的TLR2+CD14+阳性率低于二期初诊梅毒患者和隐性梅毒患者,差异有统计学意义(P<0.001);血清固定组复诊时TLR2(MFI)表达低于血清阴转组TLR2(MFI)表达(P<0.001),血清固定组的初诊和复诊者TLR2+CD14+细胞(%)均低于血清阴转组TLR2+CD14+细胞(%)(P<0.05)。结论 TLR2+CD14+阳性细胞表达上调不够,其可能导致发生梅毒血清固定。
Objective To observe the changes of Toll-like receptor 2 on the surface of peripheral blood mononuclear cells (PBMCs) before and after the treatment of syphilis. Methods The patients with newly diagnosed syphilis were collected for PBMCs TLR2 + CD14 + and TLR2 + CD14 + CD14 + cell population before and after the treatment of flooding. Sixty healthy subjects were selected as control group for comparison. Results The expression and percentage of CD14 + TLR2 + in peripheral blood of patients with syphilis at the time of first visit were significantly lower than those of the normal control group (P <0.001). The positive rate of TLR2 + CD14 + in peripheral blood of the newly diagnosed syphilis patients in one phase was lower than that in the second phase of syphilis patients and latent syphilis patients (P <0.001). TLR2 (MFI) expression in the serum fixation group (P <0.001). The TLR2 + CD14 + cells (%) in the newly diagnosed and retreated patients in the serum fixation group were lower than those in the serum negative control group (P <0.05) ). Conclusion The up-regulation of TLR2 + CD14 + positive cells is not enough, which may lead to the occurrence of syphilis serum fixation.