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目的:建立同时测定复方盐酸金霉素软膏中盐酸金霉素和盐酸丁卡因含量的方法。方法:采用双波长分光光度法,在311、368、387·5nm波长处测定其吸光度,并计算含量。结果:盐酸金霉素和盐酸丁卡因检测浓度的线性范围分别为9·90～29·71、5·99～17·98μg·mL-1(r=0·9999);平均回收率分别为99·37%(RSD=0·31%,n=9)、98·31%(RSD=0·23%,n=9)。结论:本方法操作简便、快速,结果准确,可用于该制剂的含量测定。
Objective: To establish a method for the simultaneous determination of chlortetracycline hydrochloride and tetracaine hydrochloride in compound hydrochloride chlortetracycline ointment. Methods: The absorbance was measured at the wavelengths of 311, 368, 387.5 nm by double wavelength spectrophotometry and the content was calculated. Results: The calibration curves of chlortetracycline hydrochloride and tetracaine hydrochloride were linear in the range of 9.99 ~ 29.71,5.99 ~ 17.98μg · mL-1 (r = 0.9999). The average recoveries were 99.37% (RSD = 0.31%, n = 9), 98.31% (RSD = 0.23%, n = 9). Conclusion: The method is simple, rapid, accurate and can be used to determine the content of the preparation.