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应用免疫组织化学技术 ABC法和神经节切除术 ,观察了 3 0只 Sprague-Dawley大鼠 (分正常组和四个手术组 )颈内动脉系脑底血管主要分支神经肽 Y( NPY)能神经纤维的起源。正常组大鼠脑底血管大脑前动脉、大脑中动脉和大脑后动脉均可见棕褐色的 NPY能免疫反应阳性纤维 ,呈细线状 ,攀附于血管壁上。手术 组做左侧颈上神经节切除术 ,术后存活 7～ 10 d,发现左侧大脑前动脉、大脑中动脉和大脑后动脉阳性纤维明显减少 ,右侧同名动脉阳性纤维亦有减少 ,但不如同侧明显 ;手术 组做双侧颈上神经节切除术 ,术后存活 7～ 10 d,双侧大脑前动脉、大脑中动脉和大脑后动脉阳性纤维基本消失 ;手术 组做左侧星状神经节切除术 ;手术 组做双侧星状神经节切除术 ,上述各动脉阳性纤维密度都未减少。将手术 、 组各组阳性纤维密度数据与正常组进行对比 ,经统计学分析 ,有显著差异。结果提示 :大鼠一侧颈内动脉系各主要动脉分支的 NPY能神经纤维都起源于双侧颈上神经节 ,但以同侧为主
ABC immunohistochemistry and ganglionectomy were used to observe the changes of neuropeptide Y (NPY) neurons in the blood vessels of the internal carotid artery in 30 Sprague-Dawley rats (normal and 4 surgical groups) The origin of the fiber. In the normal group, serovar NPY-positive fibers were observed in the anterior cerebral arteries, middle cerebral arteries and posterior cerebral arteries in the basal ganglia of rats, which were thinly linear and attached to the vessel wall. Surgical group did left cervical excision and survived for 7 ~ 10 days. The positive fibers of left anterior cerebral artery, middle cerebral artery and posterior cerebral artery were found to be significantly decreased, and the positive fibers of right anterior artery were also reduced Not as obvious as the ipsilateral; surgical group of bilateral cervical supratentorial ganglion surgery, postoperative survival of 7 ~ 10 d, bilateral anterior cerebral artery, middle cerebral artery and posterior cerebral artery positive fibers disappeared; operation group to do the left star Ganglionectomy; surgery group bilateral stellate ganglionectomy, the above-mentioned positive artery fiber density were not reduced. The surgery, the group positive fiber density data of each group compared with the normal group, the statistical analysis, there are significant differences. The results suggest that NPY nerve fibers of the main arterial branches of the carotid artery in one side of the rat originate from bilateral superior cervical ganglia,