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In this paper,an active optics and co-focus experimental system of segmented mirror is built.Firstly,a support structure of segmented mirror is designed and it is verified by simulation to meet the requirement for the experimental system of segmented mirror.In this system,the large de-focus and tilt/tip errors of the segmented mirror are adjusted by observing the density and contrast of interference fringes based on isoclinic interference theory until the defocus and tilt/tip errors are in the detective range of the Shack-Hartmann.Then,the Shack-Hartmann is used to measure them and they are adjusted by actuators.The actuators are controlled by active optics to realize the closed-loop adjustment and maintenance for fine co-focus of segmented mirror.And the interference fringes are utilized to verify the detective precision of ShackHartmann.After the co-focus fine-tuning of the segmented mirror,the tilt/tip residual surface error is better than 0.01λ RMS;the defocus residual surface error is better than 0.01λ RMS.