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采用荧光染色剂苯并硫羟基嘌呤(benzothiocarboxypurine,BCP)对疟原虫进行染色实验,同时用姬姆萨染色做对照。BCP染色后疟原虫厚血膜在400倍荧光显微镜下见,疟原虫各发育期及滋养体、裂殖体和配子体呈特异性苹果绿色荧光;胞桨疟色素呈黄色;白细胞胞桨染成亮绿色。血膜中的异物不呈荧光色,易区别。储存120d后的厚血膜着色效果亦较好。BCP染色恶性疟与间日疟血片,敏感性为97.4%、91.2%,特异性为66.7%、50%,阳性预测值97.6%、88.6%阴性预测值50%与40%。认为BCP染色法是一种有使用价值的疟疾诊断新方法。
The fluorescent dye benzothiocarboxypurine (BCP) was used to stain the malaria parasite, and the control was performed by Giemsa staining. Plasmodium falciparum BCP after staining at 400 times the fluorescence microscope, the developmental stages of Plasmodium and trophozoites, schizonts and gametophytes showed specific apple green fluorescence; malaria pigment cells was yellow; white blood cells paddle bright green. Foreign body blood film is not fluorescent color, easy to distinguish. After 120d of thick blood film coloring effect is also better. BCP staining of Plasmodium falciparum and Mirena malariae showed sensitivity of 97.4%, specificity of 91.2%, specificity of 66.7%, specificity of 50%, positive predictive value of 97.6%, negative predictive value of 88.6% 50% and 40%. BCP staining is considered a valuable new diagnostic method for malaria.