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目的以人参皂苷Rh_4(GRh_4)和Rk_3(GRk_3)的生成量作为考察指标,利用响应曲面法(RSM)优化GRh_4和GRk_3的制备工艺。方法利用HPLC测定GRh_4和GRk_3的量,以人参皂苷Rg1(GRg1)为底物,采用高温热裂解,对影响热裂解的酸浓度、反应温度及时间进行考察,运用RSM选择最优GRh_4和GRk_3的热裂解工艺条件。结果经HPLC测定,根据Design Expert 7.1.6软件预测经高温热裂解GRg1制备GRk_3和GRh_4的最佳工艺参数:甲酸浓度为0.02%,反应温度为116.4℃,反应时间为2.22 h;在此条件下GRk_3和GRh_4之和最大为123.56 mg,得率为61.78%。结论 RSM优化后的高温热裂解条件,适合大规模制备GRk_3和GRh_4。
OBJECTIVE To optimize the preparation of GRh_4 and GRk_3 by using response surface methodology (RSM) with ghosamine Rh_4 (GRh_4) and Rk_3 (GRk_3) production as index. Methods The amount of GRh_4 and GRk_3 were determined by HPLC, the concentration of ghrelin, the reaction temperature and time were investigated by high temperature thermal pyrolysis using ginsenoside Rg1 (GRg1) as substrate. The best GRh_4 and GRk_3 Thermal cracking process conditions. Results The optimum parameters of GRk_3 and GRh_4 prepared by high temperature pyrolysis of GRg1 were determined by HPLC using Design Expert 7.1.6 software. The concentration of formic acid was 0.02%, the reaction temperature was 116.4 ℃ and the reaction time was 2.22 h. Under these conditions The sum of GRk_3 and GRh_4 was 123.56 mg, yielding 61.78%. Conclusion RSM optimized pyrolysis conditions are suitable for large-scale preparation of GRk_3 and GRh_4.