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目的探讨周围血中ＮＫ细胞、Ｔ细胞亚群、细胞膜白细胞介素－２受体（ｍＩＬ－２Ｒ）阳性细胞与重症肌无力（ＭＧ）发病及临床疗效的关系。方法用流式细胞仪计数３９例ＭＧ患者ＣＤ３、ＣＤ４、ＣＤ８、ＣＤ２５和ＣＤ５６抗体阳性细胞的百分率，应用许氏临床评分法对病情的严重程度和转归进行评定。结果ＭＧ患者的ｍＩＬ－２Ｒ（ＣＤ２５）阳性细胞无明显改变。ＣＤ３阳性细胞在肾上腺皮素激素治疗前、后无明显改变。ＮＫ细胞（ＣＤ５６）和ＣＤ４阳性细胞在治疗前均明显高于正常（Ｐ＜００１），治疗２个月后均明显下降（Ｐ＜０．０１，０．０５）。ＣＤ８阳性细胞治疗前后明显降低和增高（Ｐ＜００５）。结论ｍＩＬ－２Ｒ阳性细胞百分率不能直接反映ＭＧ的免疫功能紊乱，并对ＮＫ细胞和Ｔ细胞亚群的变化无直接调节作用。ＮＫ细胞增高，可能为其功能下降的代偿性变化。ＣＤ４增高，ＣＤ８下降提示免疫功能紊乱，符合自身免疫性疾病的特点。
Objective To investigate the relationship between the expression of NK cells, T lymphocyte subsets and membrane-bound interleukin-2 receptor (mIL-2R) in peripheral blood and the incidence of myasthenia gravis (MG) and its clinical efficacy. Methods The percentage of CD3, CD4, CD8, CD25 and CD56 positive cells in 39 patients with MG was counted by flow cytometry. The severity and outcome of the disease were evaluated by Hsu’s clinical score. Results There was no significant change in mIL-2R (CD25) positive cells in MG patients. CD3-positive cells in the treatment of adrenaline hormone before and after no significant change. NK cells (CD56) and CD4 positive cells were significantly higher than normal before treatment (P <001), after 2 months of treatment were significantly decreased (P <0.01, 0.05). CD8 positive cells were significantly lower and higher before and after treatment (P <0 05). Conclusion The percentage of mIL-2R positive cells can not directly reflect the immune dysfunction of MG, and has no direct regulation on the changes of NK cells and T cell subsets. Increased NK cells may be compensatory changes in their decline in function. CD4 increased, CD8 decline prompted immune dysfunction, in line with the characteristics of autoimmune diseases.