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使用Ｐｒｏα１（Ⅰ）、Ｐｒｏα１（Ⅲ）人胶原ｃＤＮＡ探针，采用ｃＤＮＡ－ｍＲＮＡ班点杂交技术，观察了染尘２个月、４个月的矽肺大鼠及汉防己甲素治疗１个月和３个月的矽肺大鼠肺组织中Ⅰ型、Ⅲ型胶原的ｍＲＮＡ水平的改变情况。实验结果表明，矽肺组织中Ⅰ、Ⅲ型胶原ｍＲＮＡ的含量比正常肺组织明显增加（Ｐ＜０．０５），汉防己甲素治疗后Ⅰ、Ⅲ型胶原正常含量则较矽肺组织显著减少。因此，我们认为矽肺病变组织中胶原纤维的积聚是由石英粉尘引起胶原基因表达改变所致；汉防己甲素能直接或间接地抑制胶原基因的转录，从而抑制矽肺病变中胶原蛋白的合成。
Pro? 1 (Ⅰ) and Proα1 (Ⅲ) human collagen cDNA probes were used to observe the effects of tetrandrine and thalidomide for 1 month and 4 months after exposure to dust for 2 months and 4 months respectively Changes of mRNA expression of type I and type III collagen in the lung tissue of silicosis rats for 3 months. The results showed that the content of type I and type III collagen in silicosis increased significantly (P <0.05), and the content of type I and type III collagen in silicosis decreased significantly compared with that in silicosis. Therefore, we believe that the accumulation of collagen fibers in silicotic lesions is caused by changes in the expression of collagen genes caused by quartz dust. Tetrandrine, either directly or indirectly, inhibits collagen gene transcription and thus inhibits collagen synthesis in silicotic lesions.