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目的:观察失血对大鼠凝血纤溶系统中纤溶酶原激活物抑制物-1(plasminogen activator inhibitor-1,PAI-1)的影响。方法:将大鼠随机分为急性失血组(A组)、慢性失血组(B组)和对照组(C组),每组10只。制备动物贫血模型,采血后先检测血红蛋白(HB)并记录,血标本离心后分离血浆,ELISA法检测血浆中PAI-1的含量,共观察8 d,期间不采取任何干预措施。结果:大鼠失血、HB降低后血浆中PAI-1含量增加,其中,A组和B组PAI-1的含量较对照组差异明显,B组中随失血量的增加和HB的降低,血浆中PAI-1的含量显著增加后维持在这一高水平。A组急性大量失血后PAI-1含量先达高峰后迅速下降,随后缓慢增加,但增加的幅度不及B组。结论:大鼠血浆中PAI-1的含量随失血量的增加和HB降低而增高。
Objective: To observe the effect of blood loss on the plasminogen activator inhibitor-1 (PAI-1) in the coagulation and fibrinolytic system of rats. Methods: The rats were randomly divided into acute blood loss group (A group), chronic blood loss group (B group) and control group (C group), 10 rats in each group. The animal model of anemia was established. After the blood was collected, hemoglobin (HB) was detected and recorded. The blood samples were centrifuged and the plasma was separated. The content of PAI-1 in the plasma was detected by ELISA for 8 days. No intervention was taken during this period. Results: After the blood loss in rats and HB decreased, the content of PAI-1 in plasma increased. Among them, the content of PAI-1 in group A and group B was significantly different from that in control group. In group B, with the increase of blood loss and HB, PAI-1 content increased significantly after the maintenance at this high level. After Acute massive blood loss, the content of PAI-1 reached the peak first and then decreased rapidly, then increased slowly, but not as much as that of Group B. Conclusion: The content of PAI-1 in plasma of rats increased with the increase of blood loss and the decrease of HB.