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miRNAs是新发现的一类长约21个碱基对的非编码RNAs.它们在转录后基因调控中起着重要作用.它们的靶目标涉及许多生物进程,如发育、新陈代谢、胁迫应答等.尽管已经有大量物种的miRNAs报道,然而关于高粱miRNAs的相关报道却十分有限.本研究通过基于基因组勘测序列(GSS)的同源序列搜索和miRNAs的二级结构分析,总共发现17条新的miRNAs.它们不均等的分布于11个miRNAs家族中.一些miRNAs的基因存在多位点并且不仅仅存在于单一基因位点上.大多数miRNAs存在同一生物界的保守性,但是我们发现,高粱sbi-miR127和sbi-miR466分别表现出与人类(H.sapiens)和家鼠(M.musculus)具有高度序列相似性.通过靶基因预测软件MiRU对miRNAs的分析表明,它们可能调节64种靶基因,其中多数涉及RNA加工、新陈代谢、细胞周期、蛋白质降解、胁迫应答及转录.在11个miRNAs家族中至少有7个miRNAs的靶目标是NADPH-细胞色素p450还原酶、核苷二磷酸激酶、超氧化物歧化酶等一些在新陈代谢和胁迫应答中必要的蛋白,说明miRNAs在这些过程中起着十分重要的作用.
MiRNAs are a newly discovered class of non-coding RNAs of about 21 base pairs that play important roles in the regulation of post-transcriptional genes.They target targets involve many biological processes such as development, metabolism, stress response, etc. Although A large number of miRNAs have been reported, however, there are only a few reports of miRNAs in sorghum.In this study, 17 novel miRNAs were found by homology search based on genome sequence (GSS) and secondary structure analysis of miRNAs. They are unequally distributed in 11 miRNAs families.Many miRNAs have multiple loci and not only exist in a single locus.Most of miRNAs are conserved in the same biological community, but we found that sorghum sbi-miR127 And sbi-miR466, respectively, showed high sequence similarity to H. sapiens and M. musculus.The analysis of miRNAs by MiRU, a target gene prediction software, showed that they may regulate 64 target genes, most of which Involves RNA processing, metabolism, cell cycle, protein degradation, stress response and transcription.A target of at least seven miRNAs in the 11 miRNAs family is NADPH-cells Su p450 reductase, nucleoside diphosphate kinase, superoxide dismutase enzymes necessary in metabolism and stress response protein, indicating that miRNAs play an important role in these processes.