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目的探讨黄芪对高眼压大鼠视网膜神经节细胞(RGC)的保护作用及眼压对其保护作用的影响。方法采用烙闭大鼠双眼上巩膜静法制备高眼压大鼠模型,将SD大鼠造模后随机分为假手术组、模型组、黄芪组、点药组、联合组。造模后4周开始干预,假手术组、模型组给予生理盐水灌胃,其他组分别给予黄芪(20 g/kg)灌服、降眼压药(贝美前列素)点眼及2种措施同时干预;干预4周后,采用激光共聚焦显微镜定量分析不同组大鼠的RGC数目及凋亡率的差异。结果假手术组RGC数目明显高于其他各组(P<0.01),RGC凋亡率明显低于其他各组(P<0.01);模型组恰好与假手术组相反;点药组较黄芪组RGC数目多而凋亡率低,但均无显著性差异(P>0.05);联合组RGC数目明显高于点药组和黄芪组(P<0.05)而凋亡率明显低于此2组(P<0.05)。结论黄芪可通过抑制高眼压大鼠RGC凋亡发挥神经保护作用,与降眼压药物联合应用可提高其保护作用。
Objective To investigate the protective effect of Radix Astragali on retinal ganglion cells (RGC) in rats with ocular hypertension and the effect of intraocular pressure on its protective effect. Methods The rats were randomly divided into sham operation group, model group, Astragalus membranaceus group, point drug group and combined group. Rats in sham-operated group and model-treated group were given gavage orally at 4 weeks after operation. Astragalus mongholicus (20 g / kg) After 4 weeks of intervention, the number of RGCs and the apoptosis rate in different groups were quantitatively analyzed by laser confocal microscopy. Results The number of RGCs in sham operation group was significantly higher than that in other groups (P <0.01), and the apoptosis rate of RGC in RGC group was significantly lower than that in other groups (P <0.01) (P> 0.05). The number of RGCs in the combined group was significantly higher than that of the point-medicated group and Astragalus mongholicus group (P <0.05), and the apoptosis rate was significantly lower than that of the two groups <0.05). Conclusion Astragalus can play a neuroprotective role by inhibiting RGC apoptosis in hypertensive rats, and combined with antihypertensive drugs can enhance its protective effect.