论文部分内容阅读
目的：构建ｘｙｌＥ基因高表达菌株，为快速检测芳香类化合物污染提供可能。方法和结果：通过ＰＣＲ扩增，将位于质粒ｐＴＧ４０２上的ｘｙｌＥ基因进行扩增，克隆于ｐＵＣ１１８Ｎ和ｐＵＣ１１９Ｎ上，经双向测序，证明ＰＣＲ过程中并未发生突变，然后进一步克隆于高表达载体ｐＪＬＡ５０３，转化Ｅ．ｃｏｌｉＴＧ１，经４２℃诱导，获得了高表达的基因产物，表达量占全菌总蛋白的３４．２％。结论：ｘｙｌＥ基因在大肠杆菌中获得了高表达。
OBJECTIVE: To construct xylE gene-overexpressing strains for the rapid detection of aromatics contamination. Methods and Results: The xylE gene located in the plasmid pTG402 was amplified by PCR and cloned into pUC118N and pUC119N. The two-way sequencing proved that no mutation was found in the PCR and then cloned into the high expression vector pJLA503. Transformation E. After induction with 42 ℃, the highly expressed gene product was obtained, which accounted for 34.2% of total bacterial total protein. Conclusion: The xylE gene is highly expressed in Escherichia coli.