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为获取背根节神经元，采用Hamburger35期来亨鸡胚，摘取其脊髓腰段背根节（DRG）制成细胞悬液，并制备DRG的HE染色石蜡切片。测量DRG切片上神经元和非神经元胞体最大径后，参照细胞大小结合形态学来区分背根节细胞悬液中的神经元和非种经元成份。用离心沉降法（1000转／分，5min）和自然沉降贴壁法（37℃，30min）分离细胞悬液中的神经元。结果显示：DRG切片上神经元与非神经元（主要是卫星细胞）的脑体最大径分别在6～22pm与1～4pm之间。按此大小测得DRG细胞悬液中神经元与非神经元（主要是卫星细胞）之比约为1：12左右；经离心沉降和自然沉降贴壁后两者比值可增至约1：1．6左右。其中神经元约80％保留，而非神经元丢失约90％。表明用此技术鸡胚DRG细胞悬液中的大部分神经元被分离出来了。
To obtain dorsal root ganglion neurons, Hamburger 35 stage chicken embryos were harvested, and their spinal cord dorsal root ganglia (DRG) were harvested for cell suspension preparation. HE-stained sections of DRG were prepared. After measuring the maximum diameter of neurons and non-neuronal soma on the DRG section, neuronal and non-meiotic elements in the dorsal root ganglion cell suspension were differentiated according to the cell size and morphology. The neurons in the cell suspension were separated by centrifugal sedimentation (1000 rpm for 5 min) and native sedimentation (37 ° C for 30 min). The results showed that the maximal diameter of brain in neurons and non-neurons (mainly satellite cells) on DRG slices was between 6 ~ 22pm and 1 ~ 4pm, respectively. According to this size, the ratio of neurons to non-neurons (mainly satellite cells) in DRG cell suspension is about 1:12. After centrifugation and natural sedimentation, the ratio of the two can be increased to about 1: 1 .6 or so. About 80% of neurons are retained, while non-neuronal loss of about 90%. Indicating that most of the neurons in the suspension of chicken embryonic DRG cells have been isolated using this technique.