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目的 :报道 1例伴有t(11;2 0 ) (p15 ;q11)易位的急性单核细胞白血病 (AML -M 5 )病例及其染色体涂染、逆转录-聚合酶链反应 (RT -PCR)的研究结果。方法 :骨髓细胞经直接法或 2 4h培养法常规制备染色体标本 ,以R显带技术进行核型分析 ;以 11号和 2 0号整条染色体涂染探针进行染色体涂染 ;采用RT -PCR技术检测NUP98-TOP1融合基因的转录本。结果 :R显带和全染色体涂染的结果证实该患者染色体异常为t(11;2 0 ) (p15 ;q11) ;RT -PCR检测到NUP98-TOP1融合基因的转录本。结论 :染色体涂染和RT -PCT技术的应用有利于检出t(11;2 0 ) (p15 ;q11)。
OBJECTIVE: To report a case of acute monocytic leukemia (AML-M 5) associated with t (11; 20) (p15; q11) translocation and its chromosomal staining and RT- PCR) results of the study. Methods: Bone marrow cells were routinely prepared by direct method or 24 hours culture method, and the karyotypes were analyzed by R-banding technique. The whole chromosomes 11 and 20 were stained with chromosomal probes. RT-PCR Technology to detect transcripts of the NUP98-TOP1 fusion gene. Results: The results of R banding and whole chromosome smear showed that the chromosomal abnormality in this patient was t (11; 20) (p15; q11). The transcript of NUP98-TOP1 fusion gene was detected by RT-PCR. Conclusion: The application of chromosome painting and RT-PCR technique is good for detecting t (11; 20) (p15; q11).