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目的 研究糖尿病小鼠学习、记忆功能及糖尿病小鼠脑海马内ＮＴ－３神经元表达的变化，并观察记忆增强肽对糖尿病小鼠学习、记忆功能及脑海马内ＮＴ－３神经元表达的影响。方法 用链脲佐菌素（ｓｔｒｅｐｔｏｚｏｔｏｃｉｎ，ＳＴＺ）诱发糖尿病小鼠模型，并皮下注射记忆增强肽，４周后行水迷宫试验。水迷宫试验后对小鼠进行灌注固定，取小鼠脑海马进行冰冻切片行免疫组化染色。结果 ①记忆增强肽不影响糖尿病小鼠的血糖水平；②行为学检查（水迷宫试验）示，给予记忆增强肽的糖尿病小鼠游完全程的时间及错误反应的次数均较未给予记忆增强肽的糖尿病小鼠减少，与正常对照组相似；③神经免疫组织化学实验结果表明，记忆增强肽可阻止糖尿病小鼠海马组织内神经营养因子ＮＴ－３神经元亚群细胞表达数量的减少。结论 这些结果表明，记忆增强肽对糖尿病所致的糖尿病小鼠学习、记忆功能的减退具有改善作用，可能与海马ＮＴ－３神经元的表达正常有关。
Objective To study the changes of learning and memory ability and the expression of NT-3 neurons in diabetic hippocampus of diabetic mice and to observe the effects of memory enhancing peptide on the learning and memory function and the expression of NT-3 neurons in hippocampus of diabetic mice . Methods The diabetic mouse model was induced by streptozotocin (STZ) and the memory enhancing peptide was injected subcutaneously. After 4 weeks, the water maze test was performed. After water maze test, the mice were perfused and fixed, and the hippocampus was harvested for immunohistochemical staining. Results ① The memory enhancing peptide did not affect the blood glucose level in diabetic mice. ② The behavioral tests (water maze test) showed that the memory-enhanced peptides Diabetic mice reduced, similar to the normal control group; ③Neuronimmunohistochemistry results show that memory-enhanced peptide can prevent the decrease of the number of neurotrophic factor NT-3 neuron subpopulation cells in diabetic hippocampus. Conclusions These results indicate that memory enhancing peptide can improve the learning and memory function of diabetic mice induced by diabetes and may be related to the normal expression of NT-3 neurons in hippocampus.