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目的探讨葛根素对异丙肾上腺素诱导的小鼠心肌纤维化的保护作用及其可能机制。方法将昆明种小鼠分为溶媒对照组、心肌纤维化模型组、葛根素低、高剂量(0.6、1.2 g.kg-1)组、卡托普利(25 mg.kg-1)组。预先ig给药3 d后,同时皮下注射5 mg.kg-1异丙肾上腺素1 d后,以2.5 mg.kg-1.d-1连续注射30 d。停止注射异丙肾上腺素后,再继续ig给药7 d。称重小鼠,取心脏称重,计算心重指数(CWI);消化法测定心肌羟脯氨酸(Hydro)的含量;Masson染色观察心肌纤维化的程度并计算心肌胶原容积分数(CVF);测定小鼠心肌匀浆中超氧化物歧化酶(SOD)、丙二醛(MDA)的含量。结果葛根素给药组与模型组比较均能降低CWI和Hydro的含量;Masson染色结果显示:葛根素给药组心肌细胞间的胶原含量明显减少,CVF明显下降;与模型组比较,葛根素给药组心肌匀浆中SOD水平增高,MDA含量下降。结论葛根素对异丙肾上腺素诱导的小鼠心肌纤维化有一定的预防作用,该作用与抑制胶原的形成、抗脂质过氧化及清除氧自由基有关。
Objective To investigate the protective effect of puerarin on isoproterenol-induced myocardial fibrosis in mice and its possible mechanism. Methods Kunming mice were divided into vehicle control group, model group of myocardial fibrosis, low and high doses of puerarin (0.6,1.2 g.kg-1) group, captopril (25 mg.kg-1) group. After pre-ig administration for 3 days, the rats were injected subcutaneously with 5 mg.kg-1 isoproterenol for 1 day, then injected continuously for 2.5 days at 2.5 mg.kg-1.d-1 for 30 days. After stopping injection of isoproterenol, continue to ig administration for 7 days. The mice were weighed, the hearts were weighed, the cardiac index (CWI) was calculated, the content of hydroxyproline (Hydroxyproline) was determined by digestion, the extent of myocardial fibrosis was observed by Masson staining, and the myocardial collagen volume fraction (CVF) The contents of superoxide dismutase (SOD) and malondialdehyde (MDA) in myocardial homogenate of mice. Results Compared with the model group, the Puerarin-treated group decreased the content of CWI and Hydro. The results of Masson staining showed that the collagen content of cardiomyocytes in Puerarin-treated group decreased significantly and CVF decreased significantly. Compared with model group, In the medicine group, the level of SOD in myocardial homogenate increased and the content of MDA decreased. Conclusion Puerarin can prevent isoproterenol-induced myocardial fibrosis in mice, which may be related to the inhibition of collagen formation, anti-lipid peroxidation and scavenging oxygen free radicals.