物证精斑确证试验目前广泛首选精子检出法。检精子的方法较多,实践中大多采用酸性复红美蓝染色法,因其是一种混合染色液的复染法,操作简单,效果较好。但如遇油脂杂质较多或阴道上皮细胞较多的情况,精子着色较淡,易被掩盖,不易检出。本文介绍一种改进方法可避免上述干扰,且配制、操作简单。 Proof of evidence of spot-specific confirmation test sperm detection method is widely preferred. There are many methods of semen examination. In practice, most of them adopt acid complex red and blue dyeing method. Because it is a kind of complex dyeing method, the operation is simple and the effect is better. However, in case of more oil impurities or more vaginal epithelial cells, sperm lighter, easily covered, not easy to detect. This article describes an improved method to avoid the above interference, and the preparation, simple operation.