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本实验用增殖细胞核抗原（ＰＣＮＡ）单抗ＰＣ１０及雌激素受体（ＥＲ）多抗对７０例肝细胞性肝场组织（高分化型４９例，低分化型２１例）作免疫组织化学染色，观察不同分化肝场组织ＰＣＮＡ表达及ＥＲ与肿瘤增殖的关系。结果显示５３例肝癌组织ＰＣＮＡ阳性，其中高分化３７例（７５．５％），低分化１６例（７６．２％）．二者阳性率无明显差异，ＥＲ染色２４例阳性，包括高分化１３例（２６．５％），低分化１１例（５２．４％）．后者阳性率显著高于前者（Ｐ＜０．０５），且ＥＲ表达与ＰＣＮＡ正相关（Ｐ＜０．０５）。以上结果提示仅凭ＰＣＮＡ判断肝癌分化程度及患者预后是不妥当的；雌激素系统可能对肝细胞性肝癌增殖有促进作用。
In this experiment, 70 cases of hepatocellular liver tissue (highly-differentiated 49 cases, poorly-differentiated 21 cases) were immunohistochemically stained with PCNA monoclonal antibody PC10 and estrogen receptor (ER) polyclonal antibody. Observe the expression of PCNA and the relationship between ER and tumor proliferation in different differentiated liver fields. The results showed that 53 cases of hepatocellular carcinoma were positive for PCNA, of which 37 cases were highly differentiated (75.5%), and 16 cases were poorly differentiated (76.2%). There was no significant difference in the positive rate between the two groups. ER staining was positive in 24 cases, including 13 cases with high differentiation (26.5%) and 11 cases with poor differentiation (52.4%). The latter positive rate was significantly higher than the former (P<0.05), and ER expression was positively correlated with PCNA (P<0.05). These results suggest that PCNA alone is not suitable for judging the degree of liver cancer differentiation and patient prognosis; the estrogen system may promote the proliferation of hepatocellular carcinoma.