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从感染白叶枯病的稻叶、种子和田水中先后分离到24株噬菌体,测定了它们在琼胶平板上形成噬菌斑的大小、血清学反应、热钝化温度、铁盐的钝化作用、寄主范围、一级生长曲线以及在电镜下观察它们的形态。根据测定结果,可将它们区分为两个类群。第一群称为XOP 1型,以OP—3为代表,其噬菌斑直径为3～5毫米,热钝化温度为58℃/10分钟,易为三价铁盐所钝化;寄主范围较窄;潜育期为40分钟,上升期30分钟,平均裂解量为35个。第二群称为XOP 2型,以OP—12为代表,噬菌斑小,热钝化温度68℃/10分钟,对三价铁盐不敏感,潜育期80分钟,上升期50分钟,平均裂解量为8个。它们都是蝌蚪形,但OP—3的尾部细长而不能收缩,OP—12的尾部短粗而能收缩。
A total of 24 phage were isolated from rice leaf, seed and field water infected with bacterial leaf blight. Their size, serological reaction, heat-passivation temperature and iron salt passivation were measured on agar plates , Host range, primary growth curve and their morphology under electron microscopy. According to the results, they can be divided into two groups. The first group, named XOP 1, represented by OP-3, had a plaque diameter of 3 to 5 mm and a thermal passivation temperature of 58 ° C / 10 min, which was easily passivated by trivalent iron salts. The host range Narrow; incubation period of 40 minutes, the rise of 30 minutes, the average amount of cracking 35. The second group is called XOP 2 type. OP-12 is the representative, the plaque is small, the heat passivation temperature is 68 ℃ / 10 minutes, it is insensitive to ferric salts, the incubation period is 80 minutes, the rising period is 50 minutes, The average amount of cracking is 8. They are tadpole-shaped, but the slender tail of OP-3 does not contract, and the tail of OP-12 contracts and contracts.