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为从分子水平了解牛乳头瘤病毒(Bovine papillomavirus,BPV)新疆南疆Aks-01株全基因组序列、结构特征及遗传变异情况。本研究选取新疆南疆患病牛皮肤肿瘤样物,以乳头瘤病毒L1基因的简并引物FAP59/FAP64进行PCR法基因分型鉴定并确定其基因型,根据GenBank中BPV参考株设计扩增引物和测序引物,对Aks-01株进行全基因组扩增、测序及序列分析。序列分析表明,新疆南疆Aks-01株为BPV-2基因型,其全基因组长为7944bp,具有BPV-2基因型的结构特征,与GenBank收录的BPV-2基因型参考株核苷酸比较,同源性高达98%。与BPV-1、BPV-13的基因型参考株进化关系最近,同属Delta属。该Aks-01株为新疆南疆地区首次检测确认并测定全基因组序列的牛乳头瘤病毒。
In order to understand the whole genome sequence, structural characteristics and genetic variation of Aks-01 strain of bovine papillomavirus (BPV) in southern Xinjiang from a molecular level. In this study, the cow skin tumor samples from southern Xinjiang were selected and genotyped by PCR using the degenerate primer FAP59 / FAP64 of L1 gene of papillomavirus. The genotypes were identified by PCR, and the amplification primers were designed according to the BPV reference strains in GenBank And sequencing primers, the whole genome of Aks-01 strains were amplified, sequenced and sequenced. Sequence analysis showed that the Aks-01 strain in southern Xinjiang was a BPV-2 genotype with a total genome length of 7944bp and structural characteristics of the BPV-2 genotype. Comparing with the nucleotide sequence of the BPV-2 genotype reference strain in GenBank , Homology up to 98%. The genotypes of BPV-1 and BPV-13 have the closest phylogenetic relationship with the genus Delta. The Aks-01 strain is the first bovine papillomavirus that has been confirmed and sequenced in southern Xinjiang.