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目的:测定儿童实体恶性肿瘤多药耐药基因(mdr 1)的表达,及其产物 P-糖蛋白(P-gp)。方法:17例儿童实体恶性肿瘤组织,采用逆转录-多聚酶链反应(RT-PCR)定量检测其 mdr1mRNA 表达水平。其中15例同时应用 APAAP 免疫酶标组化法测定其 P-gp。结果:实验显示mdr1 mRNA 阳性率为82.4%(14/17),P-gp 阳性率为80.0%(12/15)。两者阳性符合率为86.7%(13/15),四格表精确概率计算两者差异无显著性意义(P>0.05)。提示 mdr1 过量表达是引起儿童实体恶性肿瘤细胞多药耐药性的重要机理。结论:mdr1 mRNA 及 P-gp 的检测,对指导临床医生预测儿童实体恶性肿瘤细胞对化疗药物的反应性、评估疗效及预后有重要意义。
Objective: To determine the expression of multidrug resistance gene (mdr 1) and its product P-glycoprotein (P-gp) in childhood solid tumors. Methods: Twenty-seven children with solid malignant tumors were collected and their mdr1 mRNA expression levels were quantitatively determined by reverse transcription-polymerase chain reaction (RT-PCR). Among them, 15 cases of P-gp were also detected by APAAP immunohistochemistry. Results: The positive rate of mdr1 mRNA was 82.4% (14/17) and the positive rate of P-gp was 80.0% (12/15). The positive coincidence rate between the two was 86.7% (13/15). There was no significant difference between the two methods (P> 0.05). Tip mdr1 overexpression is an important mechanism of causing multidrug resistance of solid tumor cells in children. Conclusion: The detection of mdr1 mRNA and P-gp is of great importance to guide clinicians in predicting the response of solid tumor cells to chemotherapeutic drugs in children and assessing the curative effect and prognosis.