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目的:探讨阿司匹林对人γδT细胞杀伤消化道肿瘤细胞株的影响。方法:用异戊烯焦磷酸法体外扩增人外周血γδT细胞。用不同浓度的阿司匹林诱导γδT细胞和消化道肿瘤SGC-7901、SW-1990、SW-480、SW-1116、LOVO细胞株,用乳酸脱氢酶法测定γδT细胞的杀伤活性,用流式细胞术(FCM)检测阿司匹林诱导前后的γδT细胞和SGC-7901、SW-1990、SW-480、SW-1116和LOVO细胞凋亡百分率。结果:γδT细胞培养10d时从扩增前4.21%增加到70.35%。阿司匹林0.4～0.8mmol/L诱导24h后的γδT细胞对5种肿瘤细胞的杀伤活性最高,浓度超过3.2mmol/L时杀伤活性呈下降趋势;SGC-7901、SW-1990、SW-480、SW-1116和LOVO细胞经不同浓度的阿司匹林诱导24h后除药物浓度在0.4mmol/L时γδT细胞对SW-480、SW-1116和LOVO细胞株的杀伤活性略有增强外,其他组与对照组比较无明显变化。3.2mmol/L阿司匹林对γδT细胞诱导24h的凋亡率(52.71%)明显高于SGC-7901、SW-1990、SW-480、SW-1116和LOVO细胞株(分别为7.88%、8.89%、6.21%、4.47%和%3.67)。结论:阿司匹林在临床常规使用的药物浓度时可增强γδT细胞杀伤肿瘤细胞作用,超过这一浓度可明显抑制γδT细胞的增殖能力和杀伤活性及增加γδT细胞的凋亡率,而对SGC-7901、SW-1990、SW-480、SW-1116和LOVO细胞株作用不明显。
Objective: To investigate the effect of aspirin on human γδT cell killing tumor cell lines. Methods: Human peripheral blood γδT cells were expanded in vitro by isopentenyl pyrophosphate method. The cytotoxicity ofγδT cells and the gastrointestinal tumor SGC-7901, SW-1990, SW-480, SW-1116 and LOVO were induced by different concentrations of aspirin. The cytotoxicity ofγδT cells was determined by lactate dehydrogenase (FCM) were used to detect the apoptosis percentage of γδT cells and SGC-7901, SW-1990, SW-480, SW-1116 and LOVO cells before and after aspirin induction. Results: The γδT cells increased from 4.21% to 70.35% at 10 days after culture. The cytotoxicity of γδT cells induced by aspirin 0.4 ~ 0.8mmol / L for 24h was the highest, and the cytotoxicity decreased when SGC-7901, SW-1990, SW-480 and SW- The cytotoxicity of γδT cells to SW-480, SW-1116 and LOVO cell lines was slightly increased after treatment with different concentrations of aspirin for 24 hours and 11mmol / L, compared with the control group obvious change. 3.2mmol / L aspirin induced apoptosis of γδT cells 24h (52.71%) was significantly higher than SGC-7901, SW-1990, SW-480, SW-1116 and LOVO cell lines (7.88%, 8.89%, 6.21 %, 4.47% and% 3.67). Conclusion: Aspirin can enhance the effect of γδT cells killing tumor cells when the concentration of aspirin is commonly used clinically. Exceed this concentration, the inhibitory effect of γδT cells on proliferation and cytotoxicity and the increase of γδT cells’ The effect of SW-1990, SW-480, SW-1116 and LOVO cell lines was not obvious.