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以干旱敏感的谷子品种‘狼尾巴’为试验材料,在营养液栽培模式下,采用20%聚乙二醇(PEG-6000)模拟干旱,研究了外源亚精胺(Spd)对干旱胁迫下谷子幼苗光合特性、碳水化合物积累以及蔗糖、淀粉合成关键酶活性的影响。结果表明,干旱胁迫显著降低了谷子幼苗光合作用和碳水化合物积累,抑制植株的发育,而外源Spd处理能在一定程度上逆转这种效果,0.1mmol/L亚精胺处理后,谷子幼苗光合参数、叶绿素荧光参数均比干旱胁迫处理显著升高,同时蔗糖磷酸合成酶、淀粉合成酶等酶活性升高,并最终促进了蔗糖、可溶性总糖以及淀粉等碳水化合物的积累,缓解了干旱胁迫对谷子幼苗生长发育的抑制。以上结果表明,干旱胁迫下,外源Spd能缓解叶片气孔的关闭,促进植株对CO2的吸收,提高植物的光合作用和碳水化合物积累能力,并最终提高了谷子幼苗的抗旱性。
Using drought-sensitive millet ’Wolf’s Tail’ as test material, 20% polyethylene glycol (PEG-6000) was used to simulate drought in nutrient solution cultivation mode. The effect of exogenous spermidine (Spd) Photosynthetic Characteristics, Accumulation of Carbohydrate, and Activities of Key Enzymes Related to Sucrose and Starch Synthesis in Millet Seedlings. The results showed that drought stress significantly reduced the photosynthesis and carbohydrate accumulation in millet seedlings and inhibited plant development, while exogenous Spd treatment could reverse this effect to a certain extent. After treatment with 0.1 mmol / L spermidine, Parameters and chlorophyll fluorescence parameters were significantly higher than those under drought stress. At the same time, the activities of sucrose phosphate synthase and starch synthase increased, which eventually promoted the accumulation of sucrose, soluble total sugar and starch and other carbohydrates, relieved the drought stress Inhibition of growth and development of millet seedlings. The above results show that under drought stress, exogenous Spd can alleviate leaf stomatal closure, promote plant absorption of CO2, enhance plant photosynthesis and carbohydrate accumulation, and ultimately improve the drought resistance of millet seedlings.