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目的:调查安徽省宣城市宣木瓜丛枝菌根(AM)真菌资源。方法:用碱解离、酸性品红染色法处理根样并研究其浸染状况;用湿筛沉淀法分离宣木瓜根际土壤中的丛枝菌根真菌孢子并进行孢子计数和种类鉴定。结果:宣木瓜可被AM真菌浸染形成典型AM;初步分离鉴定出隶属4属的18种AM真菌,其中球囊霉属Glomus11种,无梗囊霉属Acaulospora3种,盾巨孢囊霉属Scutellospora3种,内养囊霉属Entrophospora1种。结论:宣木瓜根际土壤AM真菌资源较为丰富,其中球囊霉属Glomus真菌为宣木瓜根际土壤中AM真菌的优势类群。
Objective: To investigate the arbuscular mycorrhizal (AM) fungi resources in Xuancheng, Anhui Province. Methods: The arbuscular mycorrhizal fungi spores were isolated by alkali dissociation and acid fuchsin staining. The arbuscular mycorrhizal fungi spores in the rhizosphere soil were isolated by wet sieving. The spore counts and species identification were performed. Results: 18 species of AM fungi belonging to 4 genera were isolated and identified from AM fungi. Among them, 11 species of Glomus, 3 species of Acaulospora, 3 species of Scutellospora, , Within the Saccharomyces Entrophospora species. Conclusion: Arbuscular mycorrhizal fungi (AMF) resources in the rhizosphere soil of Quercus mongolica are abundant. Among them, Glomus fungus is the dominant group of AM fungi in the rhizosphere soil of.