论文部分内容阅读
目的探讨三种不同的血清学检测方法在梅毒诊断中的运用。方法使用上海荣盛甲苯胺红不加热血清反应素试验(TRUST)试剂盒、中山生物梅毒螺旋体抗体酶联免疫吸附试验(TP-ELISA)试剂盒、日本富士梅毒螺旋体明胶颗粒凝集试验(TPPA)试剂盒对110例梅毒患者(其中一期梅毒血清22例,二期梅毒血清69例,潜伏期梅毒19例),健康体检人群200例的血清标本进行梅毒抗体检测。结果110份标本三种方法TRUST、TP-ELISA、TPPA检出率分别为87.3%、96.4%、96.4%,其中一期梅毒的检出率为72.7%、95.2%、90.9%,二期梅毒的检出率为95.5%、100%、100%,潜伏期梅毒的检出率为73.7%、84.2%、89.5%;TRUST、TP-ELISA、TPPA分别检测200份正常人标本,其特异性为96.5%、99.5%、100%。结论TP-ELISA法对各期梅毒都有较高的灵敏度和特异性,可实现自动化和标准化,适用于大批量标本的检测,且与TPPA试验的符合率高,可作为首选方法。对阳性患者再做TRUST试验,以观察判断疗效、复发及再感染。
Objective To investigate the application of three different serological tests in the diagnosis of syphilis. Methods The TRUST kit, TP-ELISA kit and TPPA kit of Shanghai Rongsheng Toluidine Red Unheated Serum Reactive Protein Assay (TRUST) Cases of syphilis were detected in 110 cases of syphilis (including 22 cases of primary syphilis, 69 cases of secondary syphilis, 19 cases of latent syphilis) and 200 cases of healthy subjects. Results The detection rates of TRUST, TP-ELISA and TPPA in the 110 specimens were 87.3%, 96.4% and 96.4% respectively. The detection rates of the first stage syphilis were 72.7%, 95.2% and 90.9% The detection rates of syphilis in incubation period were 73.5%, 100%, 100%, 73.7%, 84.2%, 89.5% respectively. The specificity of TRUST, TP-ELISA and TPPA were 96.5% , 99.5%, 100%. Conclusion The TP-ELISA method has high sensitivity and specificity for all stages of syphilis and can be automated and standardized. It is suitable for the detection of large quantities of specimens and has high coincidence rate with TPPA test. TRUST test positive patients again to observe the efficacy of judgment, recurrence and re-infection.