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目的：提取较纯的ｈＴＰＯ，为甲状腺激素合成过程的研究和建立ｈＴＰＯＡｂ检测方法提供了实验材料。方法：本文采用胰蛋白酶和脱氧胆酸钠处理手术切除人甲状腺组织，经超速离心和ＳｅｐｈａｃｒｙｌＳ－３００柱层析提纯人甲状腺过氧化物酶（ｈＴＰＯ），并进行了愈创木酚氧化法ｈＴＰＯ酶活性测定。结果：本研究获得的ｈＴＰＯ比生物活性为８．４３愈创木酚单位（ＧＵ）／ｍｇ。经ＳＤＳ－ＰＡＧＥ电泳鉴定示提纯的ｈＴＰＯ在分子量１０７ｋＤ处有一清晰色带，而于９３ｋＤ处也有一色带，但较浅。结论：该方法提纯ｈＴＰＯ操作简便、快速，获得的ｈＴＰＯ较纯。
OBJECTIVE: To extract relatively pure hTPO and provide experimental materials for the study of thyroid hormone synthesis and the establishment of hTPOAb detection method. Methods: Human thyroid tissue was excised by trypsin and sodium deoxycholate. Human thyroid peroxidase (hTPO) was purified by ultracentrifugation and Sephacryl S-300 column chromatography. The effects of guaiacol oxidase hTPO enzyme Activity assay. Results: The specific bioactivity of hTPO obtained in this study was 8.43 guaiacol units (GU) / mg. The purified hTPO was identified by SDS-PAGE electrophoresis as a clear band at a molecular weight of 107 kD and a band at 93 kD, but lighter. Conclusion: This method is simple and rapid to purify hTPO. The obtained hTPO is more pure.