目的：探讨 p16、细胞周期蛋白 D1（ cyclinD1）和 pRb在头颈部鳞癌（ head and neck squamous cell carcinoma, HNSCC）的表达及他们的相互关系和其意义。方法：应用免疫组织化学方法检测 32例原发 HNSCC患者癌组织中 p16、 cyclinD1和 pRb的蛋白表达。结果： p16、 cyclinD1、 pRb的总异常率为 90.6％（ 29/32），其中 p16和 pRb蛋白表达缺失分别为 62.5％（ 20/32）和 34.4％（ 11/32）， cyclinD1过表达为 34.4％（ 11/32）。 p16与 pRb呈负相关性（ P Objective: To investigate the expression of p16, cyclinD1 and pRb in head and neck squamous cell carcinoma (HNSCC) and their relationship and significance. Methods: The expression of p16, cyclinD1 and pRb in 32 cases of primary HNSCC were detected by immunohistochemistry. Results: The overall abnormal rate of p16, cyclinD1, and pRb was 90.6% (29/32). The loss of p16 and pRb protein expression was 62.5% (20/32) and 34.4% (11/32), respectively. The cyclinD1 overexpression was 34.4. %( 11/32). P16 negatively correlated with pRb (P