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目的:观察色胺酮对结扎冠状动脉所致心肌缺血大鼠心功能、心肌病理和心肌中COX-2的影响。方法采用结扎冠状动脉左前降支致心肌缺血模型,色胺酮0.10、0.20、0.30g/kg灌胃给药21天后,观察色胺酮对心肌缺血大鼠心功能、心肌病理和心肌组织COX-2蛋白的影响。结果①色胺酮0.10g/kg对心肌缺血大鼠的心脏功能有明显的改善作用,能增强心肌收缩力,改善心肌舒张的顺应性,表现为左心室内收缩压、左心室内压最大变化速率±dp/dtmax明显升高,与模型组比较有明显差异(P<0.05);②0.10g/kg色胺酮对结扎冠状动脉所致心肌缺血大鼠能明显减轻心肌变性,心肌纤维增生和淋巴细胞浸润;③色胺酮明显抑制缺血心肌组织中COX-2蛋白,其抑制作用随剂量的增大而逐渐增强。结论0.10g/kg色胺酮对大鼠结扎冠状动脉所致心肌缺血损伤具有明显的保护作用。
Objective: To observe the effect of histamine on the cardiac function, cardiac pathology and the expression of COX-2 in myocardium of rats with myocardial ischemia induced by ligation of coronary artery. Methods Ligation of left anterior descending coronary artery ligation to myocardial ischemia model, tryptopone 0.10, 0.20, 0.30g / kg intragastric administration of 21 days, observe the effect of histamine on cardiac function, myocardial pathology and myocardial tissue Effect of COX-2 protein. Results ① Secretion of tryptophan 0.10g / kg significantly improved cardiac function in rats with myocardial ischemia, which could enhance myocardial contractility and improve diastolic compliance, showing left ventricular systolic pressure and maximal left ventricular pressure The rate of change ± dp / dtmax was significantly higher than that of the model group (P <0.05). ②0.10g / kg of trypsin could obviously reduce the myocardial degeneration, myocardial fibrosis Proliferation and lymphocytic infiltration. (3) Secretion of cyclooxygenase-2 (COX-2) in ischemic myocardium was significantly inhibited by trypsin. The inhibitory effect increased gradually with the increase of dose. Conclusion Secretion of 0.10g / kg tryptamectin has a protective effect on myocardial ischemia induced by ligation of coronary artery in rats.