【摘 要】
：
光敏色素互作因子(phytochrome-interacting factors,PIFs)是具有基本螺旋-环-螺旋(bHLH)结构域的转录因子,它们与光敏色素相互作用,在光信号转导过程发挥重要作用.基于转录组数据注释及蛋白同源比对,共筛选出28个钩藤PIF家族成员(UrPIFs),编码蛋白的长度在420～741个氨基酸之间,理论分子量46.47～79.46 kDa;等电点5.97～9.15.亚细胞定位预测结果显示UrPIFs均位于细胞核.系统进化关系和保守基序分析表明,28个UrPIFs分为4组,都具有
【机 构】
：
贵州大学生命科学学院/山地植物资源保护与种质创新教育部重点实验室,贵阳550025;贵州省农业科学院蚕业研究所,贵阳550006;贵州大学生命科学学院/山地植物资源保护与种质创新教育部重点实验室,贵阳
论文部分内容阅读
光敏色素互作因子(phytochrome-interacting factors,PIFs)是具有基本螺旋-环-螺旋(bHLH)结构域的转录因子,它们与光敏色素相互作用,在光信号转导过程发挥重要作用.基于转录组数据注释及蛋白同源比对,共筛选出28个钩藤PIF家族成员(UrPIFs),编码蛋白的长度在420～741个氨基酸之间,理论分子量46.47～79.46 kDa;等电点5.97～9.15.亚细胞定位预测结果显示UrPIFs均位于细胞核.系统进化关系和保守基序分析表明,28个UrPIFs分为4组,都具有活性光敏色素B结合(active phytochrome B-binding,APB)结构域和bHLH结构域,而且每组成员之间具有相似的保守基序.组织表达谱显示多数UrPIFs在叶中表达量较高,其次是茎,最低的是根.在遮光条件下,除UrPIF05外,7个UrPIFs表达量呈上升趋势;转移至正常光照条件后,8个UrPIFs的表达量均呈下降趋势.尤其是UrPIF15对光较为敏感,可能在光信号转导中发挥重要作用.UrPIFs的鉴定和分析可为进一步构建钩藤光调控网络提供参考.
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