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目的:研究云南鼠尾草茎叶的化学成分,寻找抗血管生成活性成分,综合开发药用植物资源。方法:利用溶剂超声提取,硅胶柱色谱、凝胶柱色谱、MCI柱色谱等分离手段进行分离纯化,根据化合物理化性质和波谱数据对其进行结构鉴定,通过鸡胚尿囊膜模型(CAM)进行活性筛选。结果:从提取物中共分离得到7个化合物,分别被鉴定为(+)桉油烯醇(1)、5,7,4’-三羟基二氢黄酮(2)、β-香树素(3)、3β-羟-12-乌苏烯(4)、马斯里酸(5)、熊果酸(6)、3-氧-12-烯-28-乌苏酸(7)。抗血管生成实验表明化合物5,6在鸡胚尿囊膜模型中表现出较好抗血管生成作用。结论:化合物1,2,5,6首次从该植物中分离得到,并首次发现化合物5(齐墩果烷型),6(乌苏烷型)在0.8 nmol/egg的剂量下就具有抑制血管生成的活性,且呈剂量依赖性。
Objective: To study the chemical constituents of Stems and Leaves of Sage, look for the anti-angiogenic active ingredients, and develop medicinal plant resources comprehensively. Methods: The constituents were isolated and purified by ultrasonic solvent extraction, silica gel column chromatography, gel column chromatography and MCI column chromatography. The structures of the compounds were identified by physicochemical properties and spectral data of the compounds. Chick embryo allantoic membrane (CAM) Active screening. RESULTS: Seven compounds were isolated from the extracts and identified as (+) eucalyptol (1), 5,7,4’-trihydroxyflavone (2), β- ), 3β-hydroxy-12-uvaulphone (4), masriolic acid (5), ursolic acid (6) and 3-oxo-12-ene-28-ursolic acid (7). Antiangiogenic experiments showed that compounds 5 and 6 showed better anti-angiogenic effects in chick embryo chorioallantoic membrane model. CONCLUSION: Compounds 1, 2, 5 and 6 were isolated from this plant for the first time and it was first found that compounds 5 (oleanane type) and 6 (ursuzu type) Generated activity, and in a dose-dependent manner.