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目的:通过多方面评价探索建立一种反流性食管炎(RE)混合反流的病证结合的大鼠模型。方法:雄性SD大鼠随机分为假手术组、模型组、病证结合模型组,每组12只。模型组采用食管十二指肠侧侧吻合术造模,病证结合模型组采用食管十二指肠侧侧吻合术叠加夹尾刺激法的方法,术后通过行为学观察及测量体质量、进食量、饮水量、抓力等综合评价,并对死亡大鼠随即进行尸体解剖;存活动物分别于术后7、14、21d处死进行食管病理组织观察,判断该模型是否成功,并确定模型建立周期。结果:假手术组中麻醉意外死亡3只。术后非预期死亡12只(33.33%),其中模型组死亡6只(16.67%),病证结合模型组死亡6只(16.67%)。尸解分析死亡原因感染、窒息、出血、吻合口瘘和穿孔等。各组存活大鼠均可见不同程度病变形成,且术后生活质量良好。术后第3周,模型组及病证结合模型组大鼠进食量、饮水量、体质量、抓力等方面较假手术组明显下降(P<0.01,P<0.05),食管HE染色切片显示中性粒细胞浸润、黏膜基底层增厚,食管炎性反应形成。结论:食管十二指肠侧侧吻合术叠加夹尾刺激法所形成的大鼠RE肝胃不和证动物模型制备方法,模型质量及病变形成率高,术后动物并发症及死亡率相对较小。
OBJECTIVE: To explore a multidisciplinary approach to establish a rat model of combination of reflux syndrome with reflux reflux esophagitis (RE). Methods: Male Sprague-Dawley rats were randomly divided into sham-operation group, model group and syndrome-combined model group, with 12 rats in each group. The model group was established by esophageal duodenal lateral anastomosis. The model of combination of disease and syndrome was treated by superposition of tail-stimulating method with esophageal duodenum lateral anastomosis. The postoperative behavioral observation and measurement of body weight, Volume, water intake, grasping force and other comprehensive evaluation, and the dead rats were immediately autopsied; surviving animals were sacrificed at 7, 14, 21d after esophageal histopathological observation to determine the success of the model, and to determine the model to establish the cycle . Results: Three rats died of anesthesia in sham operation group. There were 12 (33.33%) unplanned deaths after operation, including 6 deaths (16.67%) in the model group and 6 deaths (16.67%) in the disease combination syndrome model group. Autopsy analysis of death causes of infection, suffocation, bleeding, anastomotic fistula and perforation. Survival rats in each group were seen varying degrees of lesion formation, and postoperative quality of life is good. At the third week after operation, the food intake, water intake, body weight and gripping power of the model group and disease-syndrome group were significantly decreased compared with the sham group (P <0.01, P <0.05) Neutrophil infiltration, mucosal basal layer thickening, esophageal inflammatory response formation. Conclusions: The preparation method of animal model of liver failure and liver syndrome induced by superposition of tail tails stimulated by esophageal duodenum lateral anastomosis, high quality of the model and high incidence of pathological changes, complication and mortality of postoperative animals small.