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The aim of our study was to determine the underlying mechanism of miR-210 on regulation of the cellcycle in nasopharyngeal carcinoma cellline CNE-1, particularly through regulation of cyclin D1, under hypoxic conditions. Methods: The CNE-1 cellline was induced with hypoxia, and the expression levels of endogenic miR-210 and cyclin D1 were detected by real-time PCR and Western blotting. Next, the luciferase assay was used to confirm that cyclin D1 is a target gene for miR-210. cellcycle and cellproliferation were detected in CNE-1 cels that were cultured under hypoxic conditions with either overexpression or knockout of miR-210 using flow cytometry and MTT assay, respectively.Results: Hypoxia induced the expression of miR-210, resulting in reduced mRNA and protein levels of cyclin D1 and repression of cyclin D1 in CNE-1 cels. Further analysis indicated that miR-210 directly binded to the 3’UTR of the cyclin D1 gene, thus regulated the expression of cyclin D1. The flow cytometry assay showed that, under hypoxic conditions, miR-210 blocked CNE-1 cels in the G1 phase, and miR-210 also inhibited the proliferation of CNE-1 cels.Conclusion: Under hypoxic conditions, miR-210 directly reduced the expression of cyclin D1, leading to CNE-1 cels blocked in G1 phase.