论文部分内容阅读
目的研究绵羊李斯特菌(Li)静脉接种和滴鼻接种C57BL/6J小鼠后,细菌在小鼠体内感染增殖规律及诱导的细胞免疫应答水平,为Li作为一种新型疫苗载体提供科学依据。方法采用寇氏法测定Li静脉接种和滴鼻接种C57BL/6J小鼠的半数致死剂量(LD_(50));以0.1×LD50剂量静脉或滴鼻接种小鼠,测定不同时间点小鼠肝、脾和肺中的细菌载量、组织病理学改变,以及肝和脾组织匀浆液中IFN-γ、肺组织匀浆液中IFN-γ、TNF-α和IL-6的含量。结果Li静脉接种和滴鼻接种小鼠的半数致死剂量分别为6.3×10~6(cfu)和2.5×10~8(cfu)。Li静脉感染小鼠后能够在肝、脾和肺中增殖,引起组织细胞坏死和炎性浸润并诱导IFN-γ分泌水平上调,三种器官中肝的各指标变化最显著。Li滴鼻感染小鼠后主要在肺部增殖,引起肺部病理损害并诱导肺组织IFN-γ、TNF-α和IL-6分泌水平上调。结论静脉接种Li可以在小鼠体内引起全身性多器官的病理和免疫反应,而滴鼻接种Li可以针对性地在小鼠肺部引起病理和免疫反应;Li可以作为一种新型疫苗载体。
OBJECTIVE: To study the proliferation of C57BL / 6J mice inoculated with Listeria monocytogenes (Li) and inoculated intranasally in mice, and to provide a scientific basis for Li as a novel vaccine vector. Methods C57BL / 6J mice were inoculated intraperitoneally and inoculated intraperitoneally with Kou’s method for half lethal dose (LD 50). The mice were inoculated intravenously or intranasally with 0.1 × LD50, and the liver, Spleen and lung bacterial load, histopathological changes, as well as liver and spleen homogenate IFN-γ, lung tissue homogenates of IFN-γ, TNF-α and IL-6 content. Results The median lethal doses of Li intravenous and intranasal inoculation were 6.3 × 10 ~ 6 (cfu) and 2.5 × 10 ~ 8 (cfu), respectively. Li vein infection in mice can multiply in the liver, spleen and lung, causing necrosis and infiltration of tissue necrosis and induction of IFN-γ secretion increased, the three organs of the most significant changes in the indicators of the liver. Li nasal infection in mice mainly in the lung proliferation, causing lung pathological damage and induce lung tissue IFN-γ, TNF-α and IL-6 secretion increased. Conclusion Intravenous inoculation of Li can cause systemic pathological and immune responses in mice. Li inoculation intranasally can induce pathological and immune responses in mouse lungs. Li can be used as a novel vaccine vector.