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新型冠状病毒肺炎(COVID-19)疫情以来，疾病的高传染性使确诊人数剧增，同时死亡人数也在日益增加。现全球已有200余个国家或地区出现感染病例。疾病的早诊可以有效控制疾病传播，同时使感染者得到有效的隔离和救治，提高生存率。目前，中国COVID-19的控制取得了阶段性胜利。本文对COVID-19的临床表现、实验室检查、影像学特征、分子学和血清学确诊方法及中国COVID-19诊疗方案多个修订版本诊断标准的变化进行综述，以期提高人们对该病的认识，为未来的研究提供更多的支持。“,”Since the outbreak of COVID-19, the high infectivity of the disease has caused a sharp increase in the number of confirmed cases, and the death toll has also been rising.Currently, more than 200 countries or regions around the world have cases of infection.Early diagnosis can successfully control the spread of the disease, and the infected persons can be effectively isolated and treated, thereby improving their survival rate.China has achieved a phased victory in controlling COVID-19 at present.This review analyzes the clinical manifestations, laboratory examinations, imaging features, the molecular and serological methods for definitive diagnosis of COVID-19, as well as the changes of diagnostic criteria in several amendments to China′s Novel Coronavirus Pneumonia Diagnosis and Treatment Program in the hope of improving people′s understanding of the disease and providing more support for future research.