听小骨体积细小,传统包埋法对其结构细节显示欠佳。作者对该法进行改良,达到较好观察效果。1　听小骨的取材与处理　在固定过的颅底处,打开鼓室盖,小心取出三块完整听小骨,仔细剥除其表面的软组织,经乙醇梯度脱脂,脱水,风干备用。2　听骨链的粘接　采用O-SECOND胶水,先涂一小滴 Listen to the small size of the small bone, the traditional embedding method for its structural details show poor. The author improved the law to achieve better observation effect. 1 Listen to the small bone material and treatment Fixed skull base, open the tympanic cap, carefully remove the three complete auditory ossicles, carefully stripped of the surface of soft tissue, degreased by ethanol gradient, dehydration, air-dried spare. 2 ossicular chain bonding using O-SECOND glue, apply a small drop first