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构建了由PGK启动子驱动HSV-tk基因的重组质粒pKGTK,反转录病毒载体pLNTK,并成功地转移至小鼠神经母细胞瘤NBA_2细胞内。体外实验证实NBAKGTK和NBALNTK对阿昔洛韦(acyclovir,ACV)的杀伤敏感性分别为NBA_2约100倍和500倍。应用~3HTdR掺入法检测DNA的合成能力,证实ACV能够明显抑制NBAKGTK和NBALNTK的DNA合成。共培养实验证实存在旁观者杀伤效应。
A recombinant plasmid pKGTK driven by PGK promoter and retroviral vector pLNTK was constructed and successfully transfected into mouse neuroblastoma cell line NBN-2. In vitro experiments showed that the killing sensitivity of NBAKGTK and NBALNTK to acyclovir (ACV) was about 100-fold and 500-fold higher than that of NBA 2, respectively. The ~ 3HTdR incorporation method was used to detect the DNA synthesis ability and confirmed that ACV could significantly inhibit the DNA synthesis of NBAKGTK and NBALNTK. Co-culture experiments confirmed the presence of bystander killer effect.