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目的研究P-糖蛋白(P-glycoprotein,P-gp)在吗啡耐受大鼠脑内额叶皮层、海马及下丘脑表达差异。方法雄性SD大鼠12只随机分为吗啡耐受组和生理盐水对照组,吗啡耐受组连续皮下注射吗啡10mL/kg,2次/d,连续7d,建立吗啡耐受模型;生理盐水对照组同时按10mL/kg注射生理盐水。模型建立并确认成功后处死大鼠,取出大鼠海马、下丘脑和额叶皮层组织,以逆转录聚合酶链反应分析比较两组不同脑区P-gp基因表达。结果吗啡耐受组大鼠海马和下丘脑P-gp的表达显著高于同组的额叶皮层(P<0.05),并显著高于生理盐水对照组相同脑区P-gp的表达(P<0.05);而在生理盐水对照组各脑区间P-gp的表达差异无显著性(P>0.05)。结论海马和下丘脑P-gp表达增加明显高于额叶皮层,P-gp表达在吗啡耐受大鼠各脑区之间存在显著差异。
Objective To investigate the expression of P-glycoprotein (P-gp) in the frontal cortex, hippocampus and hypothalamus in morphine tolerant rats. Methods Twelve male Sprague-Dawley rats were randomly divided into two groups: morphine tolerant group and saline control group, morphine tolerant group was injected subcutaneously morphine 10mL / kg twice a day for 7 days, and morphine tolerant model was established. Normal saline control group At the same time by 10mL / kg injection of saline. After successful establishment of the model, rats were sacrificed and the hippocampus, hypothalamus and frontal cortex of the rats were removed. The expression of P-gp gene in two groups of different brain regions was analyzed by reverse transcription-polymerase chain reaction (RT-PCR). Results The expression of P-gp in hippocampus and hypothalamus in morphine tolerant group was significantly higher than that in the same group (P <0.05), and significantly higher than that in saline control group (P < 0.05). However, there was no significant difference in the expression of P-gp in brain between normal saline group and control group (P> 0.05). Conclusions The expression of P-gp in the hippocampus and hypothalamus is significantly higher than that in the frontal cortex. P-gp expression is significantly different among brain regions in morphine tolerant rats.