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目的研究现居东莞人群人类血小板抗原(HPA)系统基因分型及其多态性分布特征,并建立本地区血小板供者基因频率数据库。方法用DNA提取试剂盒提取外周血标本中的DNA,用聚合酶链式反应-序列特异性引物(PCR-SSP)扩增HPA等位基因。结果每个样本均检测到HPA-1a、2a、4a-6a、16a基因;HPA-4a、5a、16a呈现纯合子单态性,未检测出相应的等位基因HPA-b;对于HPA-1、-6主要以a/a纯合子为多,a/a基因型频率分别是0.9750、0.9625,没有b/b纯合子出现。结论HPA血型系统多态性具有人群特征。与其他人群相比,现居东莞人群HPA血型系统中a基因频率较高,b基因频率较低,提示在现居东莞人群中由b基因不合所引起的同种免疫反应要低于其他种族人群。现居东莞人群HPA-1、-2、-3、-6系统具有多态性,HPA-2、-3抗原杂合率比较高,提示在临床上可能具有重要的免疫学意义。同时,在此次研究数据的基础上建立了现居东莞人群血小板基因频率数据库。
Objective To study the genotyping and polymorphism distribution of human platelet antigen (HPA) in Dongguan and to establish a database of gene frequency of platelet donor in this area. Methods DNA from peripheral blood samples was extracted by DNA extraction kit and HPA alleles were amplified by polymerase chain reaction-sequence specific primers (PCR-SSP). Results HPA-1a, 2a, 4a-6a and 16a genes were detected in each sample. Homozygous haplotypes were detected in HPA-4a, 5a and 16a, and the corresponding allele HPA-b was not detected. , -6 mainly a / a homozygote more, a / a genotype frequencies were 0.9750, 0.9625, no b / b homozygotes. Conclusion HPA blood group polymorphism has population characteristics. Compared with other populations, the prevalence of a gene in HPA blood group in Dongguan population is higher and the frequency of b gene is lower, suggesting that the same type of immune response caused by the b gene in Dongguan population is lower than other ethnic groups . HPA-1, -2, -3, -6 systems present in Dongguan population are polymorphic, and HPA-2, -3 antigen hybridization rate is high, suggesting that it may have important immunological significance in clinical practice. At the same time, based on the data of this study, a database of platelet gene frequency in Dongguan population was established.