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[Objective] To research the immunoregulatory function of the ethanol extracts (BRE) from RHIZOMA BISTORTAE. [Method] In the mice in vitro test, phagocytic function of mice macrophages RAW264.7 on neutral red was detected by colorimetric analysis. In the mice in vivo test, viscera index was calculated according to the weight of spleen and thymus. T lymphocyte proliferation and NK cell activity were detected by MTT method. Level of serum hemolysin in mice was detected based on the immune of chicken erythrocyte. IL-2 content in serum was measured by ELISA method. [Result] BRE could significantly enhance the phagocytic function of macrophages RAW264.7 in mice, increase the spleen and thymus weight in mice, promote the proliferation of T lymphocyte, enhance the cytotoxicity of NK cell, and increase the levels of serum hemolysin and IL-2 in mice. [Conclusion] RHIZOMA BISTORTAE ethanol extracts (BRE) had certain immunoregulatory functions.
[Objective] To research the immunoregulatory function of the ethanol extracts (BRE) from RHIZOMA BISTORTAE. [Method] In the mice in vitro test, phagocytic function of mice macrophages RAW 264.7 on neutral red was detected by colorimetric analysis. In the mice in vivo test, viscera index was calculated according to the weight of spleen and thymus. T lymphocyte proliferation and NK cell activity were detected by MTT method. Level of serum hemolysin in mice was detected based on the immune of chicken erythrocyte. IL-2 content in serum was measured by ELISA method. [Result] BRE could significantly enhance the phagocytic function of macrophages RAW264.7 in mice, increase the spleen and thymus weight in mice, promote the proliferation of T lymphocyte, enhance the cytotoxicity of NK cells, and increase the levels of serum hemolysin and IL-2 in mice. [Conclusion] RHIZOMA BISTORTAE ethanol extracts (BRE) had certain immunoregulatory functions.