以前应用放射免疫测定法已阐明了很多激素的生理作用。由于放免测定法本身的局限性,人们迄今为止一直未曾发现甲状腺中存在有降钙素基因相关肽。最近,Colin 等采用一种新的、快速酶放大的免疫测定方法证实大鼠甲状腺中含有降钙素基因相关肽(简称CGRP)。这种新方法与放射免疫测定法相比具有更高的特异性和灵敏度,重复性也很好(变异系数小于8%,与胰高血糖素等7种肽均无交叉反应)。其原理如下:将 The previous application of radioimmunoassay has elucidated the physiological effects of many hormones. Due to the limitations of the radioimmunoassay itself, no calcitonin gene-related peptide has been found in the thyroid so far. Recently, Colin et al. Used a novel, rapid enzymatic amplification immunoassay to confirm the presence of calcitonin gene-related peptide (CGRP) in the thyroid of rats. The new method is more specific and sensitive than radioimmunoassay and reproducible (less than 8% coefficient of variation with no cross-reactivity with seven peptides such as glucagon). The principle is as follows: will